P-180: The Protective Effects of Exogenous Melatonin on Nicotine-Induced Changes in Mouse Uterus and Fallopian Tube

Background: The aim of this study was to evaluate protective effects of melatonin on uterus and fallopian tube in female mice under treatment with nicotine. Materials and Methods: Adult Female mice were divided into four groups. Group A: control animals received normal saline, group B: were injected with nicotine 40 μg/kg. Group C:were injected with melatonin, 10 μg/ night. Group D:were injected with nicotine 40 μg/kg and melatonin 10 μg/night. All animals were treated 15 days intraperitoneally On the 16th day, all animals were dissected and uterus and oviducts were removed from the mice. Evaluations were made using KI-67, ER-ά for determining of proliferative activity and estrogen receptors and ELISA for assay of serum estradiol level. Results: Administration of nicotine in group B, showed a decrease in estradiol level and ER-ά numbers both in uterus and oviduct (p<0.05), Melatonin alone, were not effective on serum estradiol level however it significantly increased ER-ά numbers in uterus in compare with controls (p<0.05). Melatonin was not affective on proliferative activity of epithelial cells both in uterus and oviducts. Administration of melatonin in last group preserved histology of uterus or oviduct better and increased ER-ά numbers in uterus and oviduct in compare with nicotine group(p<0.05) . Conclusion: This study indicates nicotine impairs histology of uterus and oviduct and melatonin ameliorate them partly trough alteration in ER-ά numbers without affect on proliferative activity.